Food Additive DB

Cross-Country Regulation Comparison

Disclaimer: This database provides factual regulatory information compiled from official government sources. It does not constitute medical, nutritional, or safety advice. Regulatory status varies by country and may change. Always refer to your local regulatory authority for the most current information.

Annatto

E160b | INS 160b

Names & Identifiers

E Number
E160b
INS Number
160b
CAS Number
1393-63-1
Scientific Name
Bixin and norbixin
Japanese Name
アナトー
Also Known As
Bixin, Norbixin, Achiote, CI 75120

Regulation Status by Country

🇪🇺 EU Approved 🇺🇸 USA Approved 🇯🇵 Japan Approved 🇦🇺 AU/NZ Approved 🇨🇦 Canada Approved

European Union

Status: approved | Max level: varies by food category mg/kg

Widely permitted

View EFSA Opinion →

United States

Status: approved | Exempt from certification

Widely used in cheese, butter, and snack foods

Japan

Status: approved | Category: 既存添加物

Australia / New Zealand

Status: approved | Code: 160b

Canada

Status: approved

What is Annatto?

Classification

Function: colour
Type: natural
Source: plant-derived

Natural Occurrence

A natural orange-red pigment extracted from the seeds of the achiote tree, native to tropical regions of the Americas.

Found in: Seeds of the achiote tree (Bixa orellana)

How It's Made

Method: extraction
Extracted from the seed coat of achiote (Bixa orellana) using vegetable oil (bixin) or alkaline aqueous solution (norbixin).

Uses Beyond Food

  • Cosmetics: Used in cosmetics and body care products.
  • Pharmaceutical: Used as a colorant in pharmaceutical preparations.
  • Industrial: Used in textile dyeing.
  • Household: Traditionally used as a food colorant and body paint in Latin American cultures

Commonly Found In

Annatto is typically used in:

Candies and sweets Soft drinks and flavored beverages Baked goods and desserts Ice cream and frozen desserts Breakfast cereals Yogurt and flavored dairy products Snack foods and chips

Note: This list shows typical product categories and may vary by brand and region. Always check product labels for actual ingredients.

Safety Evaluations (Official)

JECFA (WHO/FAO)

ADI: 0–0.6 mg/kg bw/day (for bixin)
Evaluated: 2018

View JECFA Database →

EFSA (EU)

ADI: 6 mg/kg bw/day (for bixin); 0.3 mg/kg bw/day (for norbixin)
Evaluated: 2016

View EFSA Opinion →

ADI = Acceptable Daily Intake (mg per kg of body weight per day)

Research & Official Documents

Frequently Asked Questions

What is Annatto (E160b)?

Annatto (E160b) is a colour used in food products. It is natural and plant-derived. A natural orange-red pigment extracted from the seeds of the achiote tree, native to tropical regions of the Americas.

Where is Annatto found naturally?

Annatto is naturally found in Seeds of the achiote tree (Bixa orellana). A natural orange-red pigment extracted from the seeds of the achiote tree, native to tropical regions of the Americas.

What is the ADI for Annatto?

The Acceptable Daily Intake (ADI) for Annatto is 0–0.6 mg/kg bw/day (for bixin) as established by JECFA (Joint FAO/WHO Expert Committee on Food Additives). ADI represents the amount that can be consumed daily over a lifetime without appreciable health risk.

What foods contain Annatto?

Annatto is used in various food categories including Unripened cheese, Fat emulsions mainly of type water-in-oil. It is used as a colour in these products.

Is Annatto the same as Bixin?

Yes, Annatto is also known as Bixin, Norbixin, Achiote, CI 75120. These are different names for the same substance.

Related Additives

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